microbiome-host interactions for the prevention of colorectal cancer
colonbiome will study the interplay of genetic, transcriptomic, metabolomic and lifestyle factors with the colon microbiome.
We aim to perform a systems biology analysis of the interactions between microbiome and host at the genetic, transcriptomic, metabolomic and lifestyle levels.
Subjects paticipating in colorectal cancer screening will be studied. Patients with cancer or high risk adenoma will be compared with healthy controls. RNA-seq from colon mucosa is already available for healthy controls.
Shotgun metagenomics will be assessed for 50 subjects of each group, and 16S for 150 additional subjects from each group. All have a GWAS array to study genetic determinants of the microbiome.
Shotgun sequencing of stool DNA from 150 subjects (50 with CRC, 50 with high risk adenoma and 50 healthy controls). Validation with 16S microbiome in 150 additional subjects of each group. Analysis of differential OTUs and genes among groups.
Analysis of lifestyle, genetic variation and gene-expression associated to microbiome diversity.
Analysis of plasma metabolomics with an untargeted approach- Assessment of associations between metabolites, microbiome and host characteristics.
CRC risk models
Improve risk prediction models including lifestyle, genetics, microbiome and metabolomics data.
Victor Moreno Principal Investigator, MD, PhD
Ville Pimenoff co-Principal Investigator, PhD
Mireia Obon Nutritionist, PhD
Gemma Ibañez Gastroenterologist, MD, PhD
Robert Carreras Biologist, PhD
Adriana Lopez-Doriga Biostatistician, PhD
Elisabet Guinó Biostatistician
Anna Diez Biostatistician
M. Henar Alonso Biostatistician
Ferran Moratalla Bioinformatician
Joan Mas Bioinformatician
Josipa Bilic Project Manager, PhD
Research project PI-17/0092
Project funded by
Instituto de Salud Carlos III
, co-funded by FEDER funds –a way to build Europe–