colonbiome

microbiome-host interactions for the prevention of colorectal cancer

colonbiome will study the interplay of genetic, transcriptomic, metabolomic and lifestyle factors with the colon microbiome.


Project Aims

We aim to perform a systems biology analysis of the interactions between microbiome and host at the genetic, transcriptomic, metabolomic and lifestyle levels.

Data

Subjects paticipating in colorectal cancer screening will be studied. Patients with cancer or high risk adenoma will be compared with healthy controls. RNA-seq from colon mucosa is already available for healthy controls.

Shotgun metagenomics will be assessed for 50 subjects of each group, and 16S for 150 additional subjects from each group. All have a GWAS array to study genetic determinants of the microbiome.


Microbiome assessment

Shotgun sequencing of stool DNA from 150 subjects (50 with CRC, 50 with high risk adenoma and 50 healthy controls). Validation with 16S microbiome in 150 additional subjects of each group. Analysis of differential OTUs and genes among groups.

Microbiome-host interactions

Analysis of lifestyle, genetic variation and gene-expression associated to microbiome diversity.

Metabolome assessment

Analysis of plasma metabolomics with an untargeted approach- Assessment of associations between metabolites, microbiome and host characteristics.

CRC risk models

Improve risk prediction models including lifestyle, genetics, microbiome and metabolomics data.


Progress

Microbiome
50%
Systems biology
0%
Metabolome
0%
CRC risk models
0%

Research team


Victor Moreno Principal Investigator, MD, PhD
Victor Moreno
Ville Pimenoff co-Principal Investigator, PhD
Ville Pimenoff
Mireia Obon Nutritionist, PhD
Mireia Obon
Gemma Ibañez Gastroenterologist, MD, PhD
Gemma Ibañez
Robert Carreras Biologist, PhD
Robert Carreras
Adriana Lopez-Doriga Biostatistician, PhD
Adriana Lopez-Doriga
Elisabet Guinó Biostatistician
Elisabet Guinó
Anna Diez Biostatistician
Anna Diez
M. Henar Alonso Biostatistician
M. Henar Alonso
Ferran Moratalla Bioinformatician
Ferran Moratalla
Joan Mas Bioinformatician
Joan Mas
Josipa Bilic Project Manager, PhD
Josipa Bilic

Institutions



CIBEResp CIBER de Epidemiología y Salud Pública
IDIBELL Bellvitge Biomedical Research Institute
ICO Catalan Institute of Oncology
UB Universitat de Barcelona

Funding


Research project PI-17/0092

Project funded by Instituto de Salud Carlos III , co-funded by FEDER funds –a way to build Europe–